Comparative DOX Delivery Paradigms for Heat Responsive Liposomes

Summary: The key difference between the tumor models is vascular permeability. Temperature triggered drug release and simultaneous hyperthermia heating increased microvascular permeability and drug infusion by 9-fold. M. Dewhirst, NanoScale Drug Delivery and Hyperthermia, 2011. pp. 38-60

Nanoparticle Heating / Magnetic Hyperthermia

(1) Breast Cancer / (2) Cancer Research
(3) Trug Resistant Infections   (4) Stem Cell Therapy

Features:

A. Heating Power: 5.0-12.0 KW /150-450/900 kHz
B. Magnetic Power Intensity: (350-1400 Gauss / High SAR values)
C. Round Heating Coils: 40- 130 mm / Helmholtz Heating Coils
D. Hand-Portable Planar Magnetic Amplifier: 35-55 mm tissue depth penetration

TECHNOLOGY REPORT: The physiology of the tumor is one of the key factors responsible for the failure of chemotherapy: (1) High hydrostatic pressure of tumors and limited blood flow prevents drugs to easily penetrate tumor tissue rich with cancer cells. (2) Cancer cell's cumulative resistance to repetitive drug treatments. (3) Damaging effects of drug toxicity on non-targeted and healthy tissue. (4) Absence of a useful system to remotely trigger the release of the cancer drug specifically at the site of the tumor.

MAGNETIC HEATING: The success of treating cancerous tumors with magnetic heating is dependent on the mechanical aspect of the heating coil, its shape and how close the coil can be to deliver heating to the tumor. The Heat Station is fixed and useful for lab work. The Portable Unit (below) is useful for clinical work, as foot infection treatment.

HAND PORTABLE MAGNETIC HEATING DEVICE

The Magnetic Heating Device can be held over the infection for about 15-30 minutes. The bacteria bio-heat, stop spreading and die. Penetration into the foot is up to 50 mm. Healthy tissue is not affected by the magnetic field. (Refer to Separate MSI literature.) (Patent Pending: 509193483)

Treating Foot Ulcers: Foot Ulcers (DFU) begin as a minor infection. But from the start antibiotics fail to control the spread of the infection. The reasons are simple: (1) Antibiotics struggle to keep up with constantly mutating bacterial infections. (2) Bacterial Biofilm degrades and blocks the antibiotics from penetrating the infection. (3) Peripheral Vascular Disease blocks the blood flow to deliver the antibiotics. (4) Amputation is often the result.

$$ Cost Saving Treatment: Magnetic Heating penetrates easily into deep tissue infections. For 50 cents in electricity, the magnetic heating can cause the bacteria to Bio-Heat, die, and save the $20,000 - $30,000 potential cost of an amputation (+ an equal amount for post-surgery recovery and prosthetics). This is a practical treatment solution to cancel the soaring cost of foot ulcers — in the US, Singapore, China, Europe, Middle East...worldwide. (SCVS)

MAGNETIC FIELD MEASUREMENT

DATA COLLECTION: The Magnetic Field Probe can measure and display the magnetic field inside each heating coil..

TEMPERATURE MEASUREMENT

Temperature profile shows rising trajectory as soon as magnetic heating applied. Software is provided to display this on the researcher's laptop computer.

MEASURING NANOPARTICLE SOLUTIONS

Neoptix Fiber Optic Temperature Sensor is included with each system. Up to 2 sensors can be utilized and charted independently.

RESULTS: The temperature rise of the nanoparticle formula in the rabbit's bone marrow is measured. Heating is rapid to 45 C in 60 sec's. (Beijing University)

VARIABLE VOLTAGE CONTROL

ADJUSTABLE HEATING COIL VOLTAGE. DEVELOPMENT OF MEANINGFUL DATA BEYOND SMALL ANIMAL STUDY AND TO REACH HUMAN TRIALS REQUIRES A RANGE OF COIL DIAMETERS.

BY USE OF THE VARIABLE VOLTAGE FEATURE, THE LARGER COILS UP TO 110-130 mm WILL HAVE SIMILAR HEATING ENERGY OF THE SMALLER TEST COILS.

IT IS THE LARGER COILS THAT EQUATE TO THE SIZE OF THE SOME OF THE HUMAN BODY PARTS. HAVING SUFFICIENT HEATING POWER, FREQUENCY RANGE AND THE CORRECT HEATING COIL VOLTAGE ALLOWS FOR BETTER RESEARCH RESULTS.

EBIDI PETRI DISH HEATING DEVICE

The Hi-Flux Module (ABOVE) allows the use of a microscope to observe from above the heating of the nanoparticles in real time when placed in a Petri Dish.

Each Heating Coil operates at its Resonant Frequency. This is a frequency at which heating power is at maximum. The system uses Auto-Tune to scan across the frequency range of 100-450 kHz.

This shows the frequency at resonance: (380 kHz)

NANOCARRIER DRUG DELIVERY:

By using operator-controlled magnetic energy to remote-trigger the release of the nanocarrier drug at the tumor site, non-specific (healthy) tissue is protected from the toxic effects of chemo. Further, the heating of the nanoparticles and surrounding tumor tissue improves the permeability of the tumor tissue matrix, allowing the drug to more easily penetrate the tumor tissue. The net result is that the chemo therapeutic efficacy is improved, less cancer drug is required and the tumor's resistance to the drug is suppressed.

Taratola, O: Multifunctional Nanomedicine Platform for Concurrent Delivery of Chemo and Mild Hyperthermia for Ovarian Cancer Cells, International Journal of Pharmaceutics, 2013, pp. 1-40

Breast Cancer is not much different than treating infant brain cancer. The heating coil can be shaped in a Cup-Format to encompass the tissue and to be centered over the location of the tumor. Or, a PLANAR Coil can be pressed against the breast to deliver heating a short distance to the tumor.

This a demonstration of a Flat Planar coil heating into a small and focused area. Heating shown is exaggerated because heating a steel shim is very easy. Heating nanoparticles is more difficult by 1000%. But the concept is valid. The heating device can be pressed into the breast tissue and deliver the heating effect to the tumor.

SPECIFICATIONS:

• POWER LEVELS AVAILABLE: 5.0, 7.0, 10.0 and 12.0 KW
• AC VOLTAGE: SINGLE PHASE 208-240 VAC 50/60 Hz / THREE PHASE: 240-480 VAC / 20-30 AMPS
• AVAILABLE FREQUENCY RANGE: 100-500 KHZ (100-800 KHZ OPTIONAL)
• WATER COOLING: 12 LITERS/MINUTE @ 2 BAR (30 PSI)
• WATER CHILLER: 8000 BTU

DIMENSIONS

• POWER SUPPLY: 27.5" (60 cm) Length x 14.5" (35 cm) High x 21" (50m) Wide / Weight: 120 LBS. (60 kilos)
• HEAT STATION: 10" (25 cm) Wide x 12" ((30 cm) High x 18" (45 cm) Long / Weight: 40 lbs. (20 kilos)
• CABLE BETWEEN POWER SUPPLY AND HEAT STATION: 1 METER

Rear of the Cabinet: Power Disconnect Switch, Circuit Breaker, Water Inlet and Outlet, Pressure Gauge, Control Cable to Heat Station, +24 VDC Auxiliary Supply, Fiber Optic Sensors, Magnetic Field Sensors, Ethernet Port, Type J Thermocouple Socket, External Control Cable Socket, RS-232 / RS-485 Ports.

MSI AUTOMATION, INC.
8537 GREENBRIAR ST., WICHITA, KANSAS USA 67226
316-796 4110 WEB SITE: WWW.MSIAUTOMATION.COM-
E.Mail: DAVE@MSIAUTOMATION.COM

International Representatives:
Hong Kong/Shanghai: www.worldwide-china.com / Romania: www.pro-tehnic.ro
India: www.alliedscientific.net / Singapore: www.dmxproducts.com